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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Mitochondrion. 2015 Dec 1;26:33–42. doi: 10.1016/j.mito.2015.11.005

Figure 2. Mitochondrial positioning in larval muscles.

Figure 2

(A–C) TEM images of L3 muscles. (A) Mitochondria are loosely organized in the subsarcolemmal space (bracket labeled B) and appear closer together between the myofibrils (bracket labeled C). Panels B and C are higher magnification images corresponding to the regions bracketed in A. (D, D′) Confocal micrographs of a lateral view of L3 muscle stained with anti-ATPase 5α to label mitochondria (green), sarcomeric actin (red) and nuclei (blue). (E) Schematic illustration of mitochondrial distribution in a Drosophila L3 muscle cell. (F, F′) Anti-ATPase 5α immunostaining of mitochondria (green) in the subsarcolemmal plane (F) or within the myofibers (F′). Scale bars are indicated.