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Summary of purification of alkaline serine protease produced by Bacillus caseinilyticus
| Purification method | Total activity (U) | Total protein (mg/ml) | Specific activity (U/mg) | Purification fold | Yield (%) |
|---|---|---|---|---|---|
| Crude enzyme | 5.93 | 1.38 | 4.3 | 1.00 | 100 |
| Ammomium sulphate precipitation | 8.52 | 1.20 | 7.1 | 1.65 | 48 |
| Dialysis | 9.60 | 1.00 | 9.6 | 2.23 | 35 |
| DEAE cellulose column chromatography | 11.59 | 0.13 | 89.2 | 20.74 | 18 |
