Table 2. Multiplex Polymerase Chain Reaction Conditions for Detection of Class 1, 2 and 3 Integrons Among 200 Escherichia coli Isolates.

	Temperature, °C	Time, s
First Denaturation	94	240
Extension Final	72	600
Cycles: 30
Denaturation	94	50
Annealing	55	30
Extension	72	60