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. 2016 Jan 31;2016:6323086. doi: 10.1155/2016/6323086

Figure 4.

Figure 4

The viability of trophoblastic cells after Mfn2 knockdown. TEV-1 cells were transfected with 50 nM of siRNA-Mfn2 or control oligonucleotides. (a) The protein expression of Mfn2 in cells from the control group, mock group, and siRNA-Mfn2 group was detected by western blot. The results showed that the Mfn2 knockdown led to 81.33 ± 3.61% reduction of Mfn2 protein (∗ p < 0.05). (b) Trophoblastic cells from the control group, mock group, and siRNA-Mfn2 group were allowed to recover in full growth media for approximately 48 h before being incubated in low-mitogen media overnight. Then, cellular viability was assessed via an MTT assay. A significant loss of viability was observed with attenuation of Mfn2 (data are represented as the mean ± SD, ∗ p < 0.05).