Figure 2.

Long-term estradiol enhances I_KCNH2 through oestrogen receptor-α-dependent pathway. (A) Representative currents of HEK cells transfected with KCNH2 and oestrogen receptor-α cDNA (protocol in inset). I_KCNH2 increased after estradiol stimulation (60 pmol/L, 48 h). This was abolished by co-incubation with tamoxifen (200 nmol/L). Mean ± SD values for depolarization (‘step’) and repolarization (‘tail’)-induced currents are shown (B, n = 4–6 cells). (C) Acute estradiol exposure did not affect KCNH2 currents in identically transfected cells (mean ± SD, n = 10 each). E2, estradiol; ER, oestrogen receptor; tam, tamoxifen; TP, test potential; pA, pico Ampere; pF, pico Farad.