Figure 6.

Inhibition of Hsp90 function abrogates enhanced KCNH2 trafficking and ionic current. (A) Plasma membrane preparations of cells transfected with KCNH2 and ERα and exposed to estradiol stimulation (60 pmol/L, 48 h) in the presence or absence of the Hsp90 inhibitor geldanamycin (1 µg/mL). The level of KCNH2 membrane expression was greatly reduced by geldanamycin exposure. (B) KCNH2 currents were similarly reduced with geldanamycin (mean ± SD, n = 10 and 3 cells, respectively). Gelda, geldanamycin. Other abbreviations are as above.