Skip to main content
. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: Breast Cancer Res Treat. 2015 Oct 26;154(2):251–262. doi: 10.1007/s10549-015-3618-6

Fig. 3.

Fig. 3

Combination PDT promotes photokilling of SUM149 cells in MAME cultures. Optical sections through the depth of 3D structures were captured for 16 contiguous fields 24 h after therapy and reconstructed in 3D. Representative images show live cells (green, calcein AM) and dead cells (red, ethidium homodimer-1) for untreated dark control (a, d, g); light irradiation targeting mitochondria (BPD-690 nm) and lysosomes (NPe6-660 nm) at dose of 22.5 mJ/cm2 each (b, c); PDT targeting mitochondria (BPD) at dose of 22.5 mJ/cm2 and lysosomes (NPe6) at dose of 45 mJ/cm2 (e, f); and PDT targeting mitochondria (BPD) and lysosomes (NPe6) at dose of 45 mJ/cm2 each (h, i); scale bar equals 80 microns