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. Author manuscript; available in PMC: 2017 Feb 1.

Published in final edited form as: Biochem Pharmacol. 2015 Nov 28;101:54–70. doi: 10.1016/j.bcp.2015.11.026

Fig. 2 — Effects of PIO on AMPK and ACC phosphorylation as a function of concentration and time. Serum-deprived VSMCs were treated with increasing concentrations of PIO (3 to 30 μM) or vehicle control for 48 hr (A) or a fixed concentration of PIO (30 μM) for 1 to 48 hr (B). In addition, serum-deprived VSMCs were exposed to ROSI (30 μM) versus PIO (30 μM) for 24 or 48 hr (C). VSMC lysates were then subjected to immunoblot analysis using primary antibodies specific for pAMPK, pACC, or pLKB1. *p < 0.05 compared with vehicle control; n = 3.

Fig. 2 — Effects of PIO on AMPK and ACC phosphorylation as a function of concentration and time. Serum-deprived VSMCs were treated with increasing concentrations of PIO (3 to 30 μM) or vehicle control for 48 hr (A) or a fixed concentration of PIO (30 μM) for 1 to 48 hr (B). In addition, serum-deprived VSMCs were exposed to ROSI (30 μM) versus PIO (30 μM) for 24 or 48 hr (C). VSMC lysates were then subjected to immunoblot analysis using primary antibodies specific for pAMPK, pACC, or pLKB1. *p < 0.05 compared with vehicle control; n = 3.