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. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: Biochem Pharmacol. 2015 Nov 28;101:54–70. doi: 10.1016/j.bcp.2015.11.026

Fig. 4.

Fig. 4

Effects of PIO on mitochondrial membrane potential and AMP/ATP ratio in VSMCs. Serum-deprived VSMCs were treated with: (A) PIO (30 μM, 1 or 3 hr), CCCP (50 μM, 1 hr), or vehicle control followed by fluorescence microscopy analysis of mitochondrial membrane potential; or (B) PIO (30 μM, 3 hr) or vehicle control followed by LC-MS/MS MRM analysis of AMP/ATP ratio. n.s. not significant compared with vehicle control; n = 4.