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Extender unit generation for polyketide engineering. (a) Production of unnatural malonyl-CoA extender units via crotonyl-CoA reductase and subsequent installation into salinosporamide and FK506. (b) Installation of an alkyne into antimycin via a novel pathway (c) Generation and installation of aryl-CoA analogues into splenocin. (d) MatB-generated library of diverse malonyl-CoA extender units.
