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Engineering the extender unit specificity of the CDA NRPS. Mutation of lysine 278 to glutamine shifts the specificity of CdaPS3 from 3-methylglutamate/glutamate to 3-methylglutamine/glutamine, with the 3-methyl derivative being preferred. A glycine-3-methylglutamine dipeptide precursor is proteolyzed during fermentation to provide the source of 3-methylglutamine. Coupled with a host mutation, the precursor and Lys278Glu mutation furnish a novel product, CDA-10mGln.
