Figure 1. Time course of asynchronous release overlaps with that of the post‐spike after‐hyperpolarization .

A, single extracellular stimuli evoke glutamate synaptic currents with both fast synchronous and delayed asynchronous components of the EPSC. Top panel is 10 trials overlaid, bottom panel is a single trial. Stimulus artifact has been removed for clarity. B, time course of asynchronous release plotted on same time scale as panel D. C, average ± SEM of single spike voltage averaged across 11 neurons. Spikes were evoked with a 5 ms depolarizing current step. Expanded time course of the AHP from the same data set is shown in panel D. E, voltage traces of spikes evoked with a single synaptic stimulation from a representative neuron (10 trials overlaid, stimulus artifact removed for clarity). Expanded time course of the AHP and summated asynchronous EPSPs are shown in the inset. F, expanded time course of the AHP (average ± SEM) from 11 neurons where spikes were evoked with synaptic stimulation. In panel D, E inset and F, action potentials have been truncated for clarity.