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Real time PCR primer sequences for the three targeted genes and Actin.
| Gene name | JGI number | Primer sequence (5′–3′) |
|---|---|---|
| ZmMYB111 | GRMZM2G104551 | F: GAACGACGACGCCGTGATAAG |
| R: TCTTCCAAAAGCCACTTCACCAG | ||
| ZmMYB148 | GRMZM2G097636 | F: GCGGAGGGAAAGGAGTGGTG |
| R: GCCCATTGGGTACTGGTAGTCC | ||
| PAL | GRMZM2G029048 | F: CGTCAACGACAACCCGCTCA |
| R: GAGGCCGTTGTTGTAGTAGTCGTTC | ||
| Actin | GRMZM2G320797 | F: TCACTACGACTGCCGAGCGAG |
| R: GAGCCACCACTGAGGACAACATTAC | ||
