Table 4.
Detection of different E. coli H1 and H12 strains belonging to different O-serogroups by fliCH1, fliCH12 and fliCH1/H12 Real Time PCR assays.
| Serotypeb | Nos. of strains | CT-valuesafliCH1 | CT-valuesafliCH12 | CT-valuesafliCH1/H12 |
|---|---|---|---|---|
| O2:H1 | 1 | 21.1 | – | 22.2 |
| O2:K2:H1b | 1 | 21.1–24.3 | – | 22.2–22.9 |
| O4:H1 | 1 | 24.7 | – | 27.1 |
| O6:H1 | 5 | 21.1–24.3 | – | 21.9–25.3 |
| O15:H1 | 3 | 19.6–25.5 | – | 21.2–24.0 |
| O22:H1 | 2 | 19.6–22.0 | – | 22.6–24.2 |
| O25:H1 | 1 | 22.5 | – | 24.5 |
| O77:H1 | 1 | 21.8 | – | 23.1 |
| O79:H1 | 1 | 24.5 | – | 24.7 |
| O139:H1 | 11 | 16.5–23.7 | – | 15.0–24.6 |
| O149:H1 | 2 | 213–21.4 | – | 23.5–24.8 |
| ONT:H1 | 1 | 22.6 | – | 22.7 |
| Or:H1 | 1 | 20.1 | – | 21.9 |
| O1:H12 | 1 | – | 20.9 | 24.2 |
| O9:K9:H12b | 5 | – | 18.6–23.2 | 21.6–23.1 |
| O9:H12 | 4 | – | 17.0–17.9 | 16.0–22.6 |
| O11:H12 | 2 | – | 18.2–22.4 | 21.7–22.6 |
| O20:H12 | 2 | – | 15.2–16.9 | 20.4–21.1 |
| O49:H12 | 1 | – | 19.6 | 24.5 |
| O55:H12 | 2 | – | 20.1–22.8 | 22.5–22.7 |
| O79:H12 | 1 | – | 22.8 | 23.0 |
| O98:H12 | 1 | – | 22.6 | 24.3 |
| O104:H12 | 2 | – | 20.9–23.6 | 22.1–23.4 |
| O118:H12 | 3 | – | 18.0–23.1 | 17.1–23.4 |
| O136:H12 | 4 | – | 16.8–23.6 | 15.9–23.2 |
| O153:H12 | 3 | – | 18.3–20.9 | 22.2–23.2 |
| O157:H12 | 3 | – | 18.7–23.4 | 21.7–23.7 |
| ONT:H12 | 2 | – | 21.7–22.8 | 22.5–23.4 |
| Or:H12 | 2 | – | 19.2–20.7 | 23.0–23.3 |
a
Range of real time PCR cycle thresholds. Negative reactions are indicated with the “–” sign.
b
Reference strain Orskov non-motile and the fliC-genotype was detected by nucleotide sequencing of fliC PCR products.