Table 6.
Reaction of the fliCH1/H12, fliCH1 and fliCH12 real-time PCR assays with non-H1 and non-H12 strains.
| Serotype | Number of strains | Ct-valuesafliCH1/H12 | Ct-valuesafliCH1 | Ct-valueafliCH12 |
|---|---|---|---|---|
| O33:H6 | 1 | – | 20.6 | – |
| O40:H6 | 1 | – | 19.35 | – |
| O55:H6 | 1 | – | 17.2 | – |
| O63:H6 | 1 | – | 18.98 | – |
| O113:H6 | 2 | – | 19.38–19.46 | – |
| O125:H6 | 1 | – | 24.29 | – |
| O126:H6 | 1 | – | 20.17 | – |
| O127:H6 | 1 | – | 21.78 | – |
| O41:H7 | 1 | – | – | 21.11 |
| O55:H7 | 3 | – | – | 22.35–26.16 |
| O153:H7 | 1 | – | – | 18.21 |
| O157:H7 | 4 | – | – | 18.46–24.7 |
| O23:H15 | 1 | – | – | – |
| O157:H15 | 1 | – | – | – |
| O28:H28 | 1 | – | – | 18.73 |
| O91:H28 | 1 | – | – | 16.34 |
| O110:H28 | 1 | – | – | 15.53 |
| O116:H28 | 1 | – | – | 30.55 |
| O145:H28 | 1 | – | – | 14.99 |
| OX185:H28 | 1 | – | – | 17.18 |
| O51:H49 | 1 | – | 21.1 | – |
| O114:H49 | 1 | – | 20.65 | – |
| O181:H49 | 1 | – | 20.6 | – |
| O45:H31 | 1 | – | 19.44 | – |
| O179:H31 | 1 | – | – | 19.56 |
| O6:H34 | 2 | – | – | – |
| O86:H34 | 1 | – | 21.4 | – |
| O142:H34 | 1 | – | 21.99 | – |
| O145:H34 | 1 | – | – | 21.67 |
| O132:H34 | 1 | – | – | – |
| O132:H34 | 1 | – | – | 20.84 |
| O76:H41 | 1 | – | – | 20.75 |
| O17/77:H41 | 1 | – | 21.93 | – |
| O8:H45 | 1 | – | – | – |
| O121:H45 | 1 | – | 22.32 | – |
| O157:H45 | 1 | – | 24.34 | – |
| O186:[H45] | 1 | – | 20.92 | – |
| O119:[H52] | 1 | – | – | – |
| O2:H8 | 1 | – | – | – |
| O2:H25 | 1 | – | 27.15 | – |
| O2:H27 | 1 | – | – | – |
| O2:H40 | 1 | – | – | – |
| O4:H5 | 1 | – | – | – |
| O4:H16 | 1 | – | – | – |
| O6 | 2 | – | – | – |
| O6:H4 | 1 | – | 24.17 | – |
| O6:H10 | 1 | – | – | – |
| O7:H4 | 1 | – | – | – |
| O15:H2 | 1 | – | – | – |
| O15:H11 | 1 | – | – | – |
| O15:H16 | 1 | – | – | – |
| O15:H21 | 1 | – | – | – |
| O139:H4 | 1 | – | – | – |
| O139:H19 | 1 | – | – | – |
| O128:H2 | 1 | – | – | – |
| O128:H8 | 1 | – | – | – |
| O20:H9 | 1 | – | – | 23.23 |
| O20:H30 | 1 | – | – | – |
| O20:H33 | 1 | – | – | – |
| O20:NM | 1 | – | – | – |
| O55:H19 | 1 | – | – | 23.43 |
| O55:H21 | 1 | – | – | – |
| O55:H51 | 1 | – | – | – |
| O118:H2 | 1 | – | – | – |
| O118:H5 | 1 | – | – | – |
| O118:H8 | 1 | – | – | – |
| O118:H16 | 1 | – | – | – |
| O153:H14 | 1 | – | – | 20.86 |
| O153:H21 | 1 | – | – | – |
| O153:H25 | 1 | – | 28.37 | – |
a
Range of real time PCR cycle thresholds. Negative reactions are indicated with the “–” sign.