Table 2.
The relative abundance of different phylotypes from “mature” Fe(III)-oxide mats of One Hundred Spring Plain and Beowulf Spring (YNP) determined using random DNA sequencing over multiple years (and sequencing technologies), or short-fragment 16S rRNA gene sequencing (Illumina iTag).
| Spring | One Hundred Spring Plain Spring | Beowulf Spring | |||||
|---|---|---|---|---|---|---|---|
| Random | Random | 16S Tags | Random | Random | Random | 16S Tags | |
| Sequencing technology | 454 | Illumina | Illumina | Sanger | 454 | Illumina | Illumina |
| pH/Temperature (°C) | 3.6/72 | 3.5/75 | 3.5/75 | 3/65 | 2.9/66 | 2.9/68 | 2.9/68 |
| PHYLOTYPE | RELATIVE ABUNDANCE (%) | ||||||
| M. yellowstonensis | 10.3 | 16 | 0.51 | 3.9 | 6.6 | 12 | – |
| Hydrogenobaculum spp. | 3.4 | 3 | 24.2 | 2 | 1.2 | 4.3 | 14 |
| Geoarchaeota | 43.4 | 33 | 41 | 1 | 3 | 6 | 4 |
| Novel archaeal group 2 | 4 | 6 | 24 | 26 | 38 | 27 | 42 |
| Thaumarchaeota | 0.4 | 0.4 | 3 | 19 | 4.4 | 2 | 3.2 |
| Novel archaeal group 3 | 0.1 | 0.12 | – | 4 | 3 | 5 | 26 |
| Other Sulfolobales | 2.5 | 4 | 3.3 | 3.9 | 10 | 11 | 8.5 |
| Nanoarchaeota | 0.2 | 0.6 | – | 0 | 0.1 | 0.6 | – |
–, not identified, Sample dates for One Hundred Spring Plain 454 (July 15, 2010) and Illumina (October 11, 2011), and Beowulf Sanger (August 7, 2006), 454 (July 15, 2010), and Illumina (November 16, 2011).