Skip to main content
. 2016 Feb 15;7:124. doi: 10.3389/fmicb.2016.00124

Figure 4.

Figure 4

UL42 binds to importins α3 and α4 through its NLS. (A) Co-IP of HEK293T cells cotransfected with recombinant constructs encoding Flag–UL42 and HA-tagged importin α1, α3, α4, α5, α6, α7, or α8. (B) Co-IP of pCMV-Flag–UL42-transfected and untransfected HEK293T cells to identify the association between UL42 and endogenous importin α3. (C) Co-IP of pCMV-Flag–UL42-transfected and untransfected HEK293T cells to identify the association between UL42 and endogenous importin α4. (D) Co-IP of HEK293T cells cotransfected with recombinant constructs encoding Flag–UL42 or Flag–UL42ΔNLS and HA-tagged importin α3. (E) Co-IP of HEK293T cells cotransfected with recombinant constructs encoding Flag–UL42 or Flag–UL42ΔNLS and HA-tagged importin α4. IP was performed using an MAb recognizing the Flag tag, and the western blotting (WB) was probed with the antibodies indicated on the left. The cytoskeletal protein β-actin was used as the internal control. The asterisk indicates the heavy chain of IgG. The positions of the molecular mass markers (kDa) are indicated on the left. The WB results are representative of three or more independent experiments.