Figure 2.

Characterization and sphere-forming capabilities of OM-MSCs. Adherent cells migrated from the explants and most cells became spindle shaped (a), the rapidly grown cells within nuclear disintegration and cell cleavage (b), immunocytochemistry of the characteristic markers of human OM-MSCs: STRO-1 ((c) and (g)) and nestin ((d) and (h)). After cultivating in the appropriate culture conditions, as expected, cells tended to aggregate; many loosely attached or floating spheres appeared ((e) and (f)). Neurospheres can be expressed as nestin throughout the whole process; nuclei are counterstained with DAPI (blue). Scale bars: (a) 100 μm; (b) 200 μm, white box: 100 μm; (c) and (d) 50 μm; (e) and (g) 200 μm; (f) and (h) 50 μm. DAPI = 4′,6-diamidino-2-phenylindole; MSC = mesenchymal stem cell.