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. 2016 Feb 1;2016:8648356. doi: 10.1155/2016/8648356

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Copyright © 2016 Nidal Ghosheh et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Representative micrographs from immunocytochemical analysis at days 0, 5, and 7 during hepatic differentiation of the cell line AS034. Magnification: 20x. Panels (a)–(d) show staining of undifferentiated hPSC at day 0: (a) and (c) show nuclear DAPI staining, (b) shows Oct4 staining, and (d) shows SSEA-1 staining. Panels (e)–(h) show staining of DE cells at day 5 of differentiation: (e) shows nuclear DAPI staining, (f) shows Oct4 staining, (g) shows Sox17 staining, and (h) shows a merge of DAPI and Sox17 staining. Panels (i)–(l) show staining of DE cells at day 7 of differentiation: (i) shows nuclear DAPI staining, (j) shows Oct4 staining, (k) shows Sox17 staining, and (l) shows a merge of DAPI and Sox17 staining.