Table 2.
Primary antibody | Class | Dilution | Distributor, catalogue number |
Secondary antibody | Dilution | Distributor, catalogue number | Applied to cells day |
---|---|---|---|---|---|---|---|
Albumin (ALB) | Rabbit-IgG | 1 : 500 | DakoCytomation, A0001 | Donkey anti-rabbit-IgG-Alexa 488 | 1 : 1000 | ThermoFisher Scientific, A-21206 | 24, 25, 29, 30 |
CYP3A (4) | Rabbit-IgG | 1 : 200 | Cypex, PAP011 | Donkey anti-rabbit-IgG-Alexa 488 | 1 : 1000 | ThermoFisher Scientific, A-21206 | 29, 31 |
CK 18 | Mouse-IgG1 | 1 : 100 | DakoCytomation, M7010 | Goat anti-mouse-IgG-Alexa 488 | 1 : 500 | ThermoFisher Scientific, A-11029 | 24, 25, 29, 30 |
HNF4a | Rabbit-IgG | 1 : 400 | Santa Cruz, sc-8987 | Donkey anti-rabbit-IgG-Alexa 488 | 1 : 1000 | ThermoFisher Scientific, A-21206 | 14, 24, 25, 29, 30 |
Oct4 | Mouse-IgG2b | 1 : 200 | Santa Cruz, sc-5279 | Donkey anti-mouse-IgG-Alexa-488 | 1 : 1000 | ThermoFisher Scientific, A-21202 | 0, 5, 7 |
Sox17 | Goat-IgG | 1 : 500 | R&D Systems, AF1924 | Donkey anti-goat-IgG Alexa 594 | 1 : 1000 | ThermoFisher Scientific, A-11058 | 5, 7 |
SSEA-1 | Mouse-IgM | 1 : 200 | Santa Cruz, sc-21702 | Donkey anti-mouse-IgG-Alexa-488 | 1 : 1000 | ThermoFisher Scientific, A-21202 | 0 |
AAT | Rabbit-IgG | 1 : 200 | DakoCytomation, A0012 | Donkey anti-rabbit-IgG-Alexa 488 | 1 : 1000 | ThermoFisher Scientific, A-21206 | 24, 25, 29, 30 |
α-fetoprotein (AFP) | Mouse-IgG1 | 1 : 500 | Santa Cruz, sc-51506 | Goat anti-mouse-IgG-Alexa 594 | 1 : 1000 | ThermoFisher Scientific, A-11032 | 14, 24, 25, 29, 30 |
BSEP | Goat-IgG | 1 : 100 | Santa Cruz, sc-17292 | Donkey anti-goat-IgG-Alexa-488 | 1 : 1000 | ThermoFisher Scientific, A-11055 | 29 |
OATP-C (OATP1B1) | Rabbit-IgG | 1 : 200 | Santa Cruz, sc-33609 | Donkey anti-rabbit-IgG-Alexa 488 | 1 : 1000 | ThermoFisher Scientific, A-21206 | 29 |
NTCP | Goat-IgG | 1 : 400 | Santa Cruz, sc-107030 | Donkey anti-goat-IgG-Alexa-594 | 1 : 1000 | ThermoFisher Scientific, A-11058 | 29 |
MRP2 | Rabbit-IgG | 1 : 50 | Santa Cruz, sc-20766 | Donkey anti-rabbit-IgG-Alexa 488 | 1 : 1000 | ThermoFisher Scientific, A-21206 | 29 |