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. 2016 Feb 1;2016:8648356. doi: 10.1155/2016/8648356

Table 2.

Summary of primary and secondary antibodies used in the study.

Primary antibody Class Dilution Distributor,
catalogue number
Secondary antibody Dilution Distributor, catalogue number Applied to cells day
Albumin (ALB) Rabbit-IgG 1 : 500 DakoCytomation, A0001 Donkey anti-rabbit-IgG-Alexa 488 1 : 1000 ThermoFisher Scientific, A-21206 24, 25, 29, 30
CYP3A (4) Rabbit-IgG 1 : 200 Cypex, PAP011 Donkey anti-rabbit-IgG-Alexa 488 1 : 1000 ThermoFisher Scientific, A-21206 29, 31
CK 18 Mouse-IgG1 1 : 100 DakoCytomation, M7010 Goat anti-mouse-IgG-Alexa 488 1 : 500 ThermoFisher Scientific, A-11029 24, 25, 29, 30
HNF4a Rabbit-IgG 1 : 400 Santa Cruz, sc-8987 Donkey anti-rabbit-IgG-Alexa 488 1 : 1000 ThermoFisher Scientific, A-21206 14, 24, 25, 29, 30
Oct4 Mouse-IgG2b 1 : 200 Santa Cruz, sc-5279 Donkey anti-mouse-IgG-Alexa-488 1 : 1000 ThermoFisher Scientific, A-21202 0, 5, 7
Sox17 Goat-IgG 1 : 500 R&D Systems, AF1924 Donkey anti-goat-IgG Alexa 594 1 : 1000 ThermoFisher Scientific, A-11058 5, 7
SSEA-1 Mouse-IgM 1 : 200 Santa Cruz, sc-21702 Donkey anti-mouse-IgG-Alexa-488 1 : 1000 ThermoFisher Scientific, A-21202 0
AAT Rabbit-IgG 1 : 200 DakoCytomation, A0012 Donkey anti-rabbit-IgG-Alexa 488 1 : 1000 ThermoFisher Scientific, A-21206 24, 25, 29, 30
α-fetoprotein (AFP) Mouse-IgG1 1 : 500 Santa Cruz, sc-51506 Goat anti-mouse-IgG-Alexa 594 1 : 1000 ThermoFisher Scientific, A-11032 14, 24, 25, 29, 30
BSEP Goat-IgG 1 : 100 Santa Cruz, sc-17292 Donkey anti-goat-IgG-Alexa-488 1 : 1000 ThermoFisher Scientific, A-11055 29
OATP-C (OATP1B1) Rabbit-IgG 1 : 200 Santa Cruz, sc-33609 Donkey anti-rabbit-IgG-Alexa 488 1 : 1000 ThermoFisher Scientific, A-21206 29
NTCP Goat-IgG 1 : 400 Santa Cruz, sc-107030 Donkey anti-goat-IgG-Alexa-594 1 : 1000 ThermoFisher Scientific, A-11058 29
MRP2 Rabbit-IgG 1 : 50 Santa Cruz, sc-20766 Donkey anti-rabbit-IgG-Alexa 488 1 : 1000 ThermoFisher Scientific, A-21206 29