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Aconine inhibits RANKL-induced osteoclast differentiation. (A) RAW264.7 cells were cultured for 4 d with RANKL (100 ng/mL) in the presence of varying concentrations of aconine and then stained for TRAP activity. Representative photomicrographs were taken under a light microscope (magnification ×100). (B) TRAP-positive cells containing more than three nuclei were counted as osteoclasts. Mean±SEM. n=3. cP<0.01 vs control; eP<0.05, fP<0.01 vs only RANKL-treated cells.
