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Aconine inhibits the RANKL-induced mRNA expression of osteoclast-specific genes. RAW264.7 cells were pretreated with varying concentrations of aconine for 2 h and then treated with RANKL (100 ng/mL) for 24 h. The mRNA expression levels of osteoclast-specific genes were analyzed using real-time PCR. Mean±SEM. n=3. cP<0.01 vs control; eP<0.05, fP<0.01 vs only RANKL-treated cells.
