Figure 7.

Aconine decreases the expression level of the fusion molecule DC-STAMP. RAW264.7 cells were pretreated with varying concentrations of aconine for 2 h and then treated with RANKL (100 ng/mL) for 24 h. (A) DC-STAMP mRNA levels were analyzed using real-time PCR. (B) The protein levels of DC-STAMP were determined using Western blot analysis. Protein levels were quantified using densitometry. Mean±SEM. n=3. ^bP<0.05, ^cP<0.01 vs control; ^eP<0.05, ^fP<0.01 vs only RANKL-treated cells.