Table 1. Changes in expression of glucose/lipid metabolism related genes in the liver. bP<0.05, cP<0.01 vs Control.
| Gene name | Primers |
Fold changes |
||
|---|---|---|---|---|
| Right | Left | Control (n=8) | Leucine (n=9) | |
| Triglyceride metabolism | ||||
| Fasn (NM_007988) | GTTGGCCCAGAACTCCTGTA | GTCGTCTGCCTCCAGAGC | 1.54±0.57 | 1.53±0.41 |
| Scd1 (NM_009127) | CAGCCGAGCCTTGTAAGTTC | GCTCTACACCTGCCTCTTCG | 1.68±0.35 | 3.95±0.65c |
| Srebf1 (NM_011480) | TGGTTGTTGATGAGCTGGAG | GGCTCTGGAACAGACACTGG | 1.00±0.11 | 4.73±0.74c |
| ChREBP (NM_021455) | AACCACACACTGGGCTCTTC | CTTCAAAGGCCTCAAGTTGC | 0.64±0.13 | 0.13±0.02 |
| Lxralpha (NM_013839) | TGGAGAACTCAAAGATGGGG | TGAGAGCATCACCTTCCTCA | 0.84±0.08 | 1.02±0.08 |
| Lxrbeta (NM_009473) | AGAACTTGTGGGGGAAGACA | GGTGCAGTCATGAGCCCC | 0.90±0.09 | 1.10±0.07 |
| Pparg (NM_001127330) | TCTTCCATCACGGAGAGGTC | GATGCACTGCCTATGAGCAC | 1.14±0.10 | 1.75±0.27 |
| Ppard (NM_011145) | GGTCATAGCTCTGCCACCAT | ACTCAGAGGCTCCTGCTCAC | 1.11±0.37 | 2.07±0.82 |
| Pgc1a (NM_008904) | TGAGGACCGCTAGCAAGTTT | TGTAGCGACCAATCGGAAAT | 1.02±0.23 | 1.26±0.38 |
| Pgc1b (NM_133249) | GAGGTCAAGCTCTGGCAAGT | GCTCTCGTCCTTCTTCCTCA | 0.66±0.16 | 1.79±0.28c |
| Fatty acid transfer | ||||
| Slc27a1 (NM_011977) | AGCCGAACACGAATCAGAAC | TTCTGTGTGTACGTGGGTGG | 0.99±0.34 | 1.10±0.29 |
| Slc27a5 (NM_009512) | GGCCAAGGTAGAAGCAGTGA | CCAGTGTGCTGATTGTGGAT | 1.03±0.12 | 1.35±0.07 |
| Cd36 (NM_007643) | CCTGCAAATGTCAGAGGAAA | GCGACATGATTAATGGCACA | 0.65±0.14 | 0.75±0.16 |
| Fabp1 (NM_017399) | GATTTCTGACACCCCCTTGA | TGCAGAGCCAGGAGAACTTT | 0.93±0.33 | 1.29±0.22 |
| Cholesterol metabolism | ||||
| Hmgcr (NM_008255) | CACAATAACTTCCCAGGGGT | GGCCTCCATTGAGATCCG | 1.33±0.18 | 1.92±0.31 |
| Srebp2 (NM_033218) | CACAATATCATTGAAAAGCGCTACCGGTCC | TTTTCTGATTGGCCAGCTTCAGCACCATG | 0.86±0.10 | 1.33±0.21 |
| Abcg5 (NM_031884) | ACCTTACCCACGGTTCCTTTC | CGCCCTTTAGCGTGTTGTTC | 1.02±0.18 | 2.33±0.29c |
| Abcg8 (NM_026180) | AGCTGGGCATCCGAAATCTA | GTTGGGCAGCAGTTGGTCAT | 1.11±0.24 | 2.07±0.18c |
| Abcg1 (NM_009593) | TTTCCCAGAGATCCCTTTCA | ATCGAATTCAAGGACCTTTCC | 1.12±0.13 | 1.52±0.13b |
| Abca1 (NM_013454) | GCTGCAGGAATCCAGAGAAT | CATGCACAAGGTCCTGAGAA | 0.93±0.03 | 0.97±0.05 |
| Glucose metabolism | ||||
| G6pc (NM_008061) | GTGTCCAGGACCCACCAATA | ACTGTGGGCATCAATCTCCT | 0.85±0.11 | 0.34±0.09c |
| Pck1 (NM_011044) | TGTCTTCACTGAGGTGCCAG | CTGGATGAAGTTTGATGCCC | 0.66±0.18 | 1.05±0.11 |