Table 1.
List of strains and plasmids used in this study.
| Strain or plasmid | Characteristic(s)a | Source or reference |
|---|---|---|
| Strains | ||
| Escherichia coli | ||
| DH5α | F- mcrA Δ(mrr-hsdRMS-mcrBC), Φ 80 lacZ ΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara,leu) 7697 galU galK λ- rpsL nupG tonA | Invitrogen |
| Pseudomonas aeruginosa | ||
| PAO1 | Wild type, lab strain | This study |
| PAN1 | nor insertion mutant, GmR | This study |
| PA515 | Wild type, clinical strain | Clinic |
| PAN515 | nor insertion mutant, GmR | This study |
| PA196 | Wild type, clinical strain | Clinic |
| PA554 | Wild type, clinical strain | Clinic |
| PA914 | Wild type, clinical strain | Clinic |
| Plasmids | ||
| pEX18Amp | oriT+sacB+ gene replacement vector with multiple-cloning site from pUC18, AmpR | [18] |
| pHpΏ45 | Smr/Spcr gene from the R100.1 plasmid, transcription-termination sequences from pMJK4-18 plasmid, SmR/SpcR | [28] |
| pZ1918Gm | Source plasmid of Gmr cassette, GmR | [29] |
| pRK2013 | Broad-host-range helper vector, KanR | [19] |
| pEXB | pEX18Amp containing a nor fragment, AmpR | This study |
| pEXB1lacZ | pEXB1 containing Gmr-lacZ fragment from pZ1918Gm insert in the SphI site, AmpR/GmR | This study |
a
Antibiotic resistance markers: AmpR ampicillin, GmR gentamicin, KanR kanamycin.