Fig. 1.

Phylogenetic tree highlighting position of Anaerosalibacter massiliensis sp. nov. strain ND1^T relative to other type strains within Clostridiaceae. Strains and their corresponding GenBank accession numbers for 16S rRNA genes are (type = ^T): A. massiliensis strain ND1^T, HG315673; A. bizertensis strain C5BEL^T, HQ534365[3]; A. bizertensis strain M3, HG964477; S. acetigenes strain Lup33^T, NR_025151 [28]; C. ultunense strain BS^T, GQ461825[29]; T. creatinini strain BN11^T, FR749955[30]; C. hastiforme strain ATCC 33268^T, X80841[23], [31]; T. preacuta strain ATCC 25539^T, GQ461814[32]; C. acidurici strain ATCC 7906^T, M59084[23], [33]; C. aceticum strain ATCC 35044^T, Y18183[34]; C. bifermentans strain ATCC 638^T, AB075769[23], [35]; C. dakarense strain FF1^T, KC517358[36]; C. saccharobutylicum strain ATCC BAA-117^T, U16147[37]; C. butyricum strain ATCC 19398^T, AJ458420[23], [38]; C. absonum strain ATCC 27555^T, X77842[39]; C. senegalense strain JC122^T, NR_125591 [7]; C. sporogenes strain ATCC 3584^T, X68189[23], [35]; C. aciditolerans strain JW/YJL-B3^T, DQ114945[40]; C. acidisoli strain CK74^T, AJ237756[41]; Hungatella hatheweyi strain UB-B.2^T, HE603919[42]; C. bolteae strain ATCC BAA-613^T, AJ508452[43], [44]. 16S rRNA from A. massiliensis (1512 bp) was amplified and sequenced using fd1 (5′- AGAGTTTGATCCTGGCTCAG-3′) and rP2 (5′-ACGGCTACCTTGTTACGACTT-3′) primers; 16S rRNA sequences from all studied strains were aligned using CLUSTALW; total of 1182 nucleotide positions present in all studied sequences were used for phylogenetic inferences with maximum-likelihood method within MEGA6 software. Numbers at nodes are percentages of bootstrap values obtained by repeating analysis 500 times to generate majority consensus tree. Only bootstrap values greater than 70% are indicated. Acetobacterium bakii strain DSM 8239^T, X96960[45], was used as outgroup. Scale bar = 2% nucleotide sequence divergence.