Figure 2. Progeny of V. campbellii-exposed ancestors exhibit no increased resistance against challenge with heterologous Vibrio strain.

See Fig. 9 for explanation of the treatment (T) and control (C) groups. (A–C) At every generation [F1 (A), F2 (B), and F3 (C)], the ovoviviparously produced larvae (at instar II stage) were challenged with H6 at 10⁷cells/mL and survival was scored at 12 h intervals. (D–F) Cysts from generations F1 (D), F2 (E), and F3 (F), were hatched simultaneously under axenic conditions, after which the larvae were challenged with H6, and survival was scored (common garden test). Values are presented as means ± SE (n = 3). Asterisks represent significant difference between the control and treatment *(P < 0.05), **(P < 0.01), ***(P < 0.001).