FIGURE 2.

Assembly and expression of the Cysta-tag in N. benthamiana. (A) Schematic representation of gene constructs for expression of SlCYS8 and the Cysta-tag in planta, showing the relative location of poly-His motif (black areas) and flanking residues. PDI, signal peptide of alfalfa protein disulphide isomerase for targeting to the cell secretory pathway (sec). (B) Coomassie blue-stained polyacrylamide slab gel with protein extracts of leaf tissue expressing SlCYS8 and Cysta-tag in the cytosol or the secretory pathway. Protein bands for SlCYS8 and the Cysta-tag show similar steady-state levels in leaves and a slightly higher molecular weight for the Cysta-tag due to insertion of the (His)₆ motif. EV stands for protein extract from leaf tissue infiltrated with agrobacteria harboring an ‘empty’ control vector.