Figure 4. Effect of apocynin on superoxide radical production from BALF cells obtained from mice exposed to cigarette smoke (CS) and infected with influenza A virus (IAV).

BALF cells recovered from apocynin (5 mg kg⁻¹) or vehicle (0.1% DMSO) treated CS-exposed and IAV-infected mice 3 (A) and 7 (B) days post infection were assessed ex vivo for superoxide production under basal and PDBu-stimulated conditions. Data in relative light units(RLU)/second per 1000 cells are expressed as mean ± SEM for n = 9–12 mice. Statistical significance was assessed using two-way ANOVA and Bonferroni post-hoc test. *P < 0.05, ^#P < 0.001.