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. 2016 Feb 15;6:21021. doi: 10.1038/srep21021

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(A) Phase-contrast observation of rabbit limbal epithelial cells grown on DAM or UAM carrier for 2 weeks (Bar: 100 μm). (B) Optical property of tissue engineered corneal epithelium grown on DAM and UAM inserts respectively. (C) H&E stained sections of tissue engineered corneal epithelial cell sheets generated on DAM and UAM (Bar: 50 μm). (D) Representative transmission electron microscopic images of tissue engineered corneal epithelial cell sheets generated on DAM and UAM. The superficial cell layer (red insert) and basal cell layer (blue insert) were observed under different magnifications, respectively. Arrow heads in superficial layer demonstrated cell-cell junction space, and arrow heads in basal layer demonstrated basal cell-AM junction space. (E) Connexin 43, β-catenin, and Claudin-1 immunostaining showed weaker expression in DAM based epithelium, compared with that of UAM based epithelium (Bar: 50 μm).