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. 2016 Feb 15;6:20992. doi: 10.1038/srep20992

Figure 1. Conditioned medium from RAW 264.7 cells exposed to apoptotic cells reduced TGF-β1-induced EMT in lung epithelial cells.

Figure 1

RAW 264.7 cells were stimulated with apoptotic (ApoJ) or necrotic (NecJ) Jurkat cells for 20 h. Conditioned medium (CM) was added to LA-4 cells (a–e) or primary mouse alveolar type II epithelial (AT II) cells (f) in the absence or presence of 10 ng/ml TGF-β1 for 72 h. (a,f) Immunoblots of total cell lysates were performed with anti-E-cadherin, N-cadherin, or α-SMA antibodies. Right: Densitometric analysis of the indicated EMT markers’ relative abundances. (b–d) The amount of EMT markers’ mRNA in LA-4 cell samples was analyzed by real-time PCR and normalized to that of Hprt mRNA. Values represent the mean ± s.e.m. of three independent experiments. *P < 0.05; compared with control or conditioned medium from RAW cells with ApoJ, or NecJ cells at 72 h after TGF-β1 treatment; +P < 0.05 as indicated. (e) Immunofluorescence staining for E-cadherin (red) or α-SMA (green) in LA-4 cells. Scale bars = 20 μm. Results are representative of three independent experiments.