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. 2016 Feb 15;6:20992. doi: 10.1038/srep20992

Figure 7. PGE2, PGD2, and HGF secretion from murine BMDM mediates EMT reduction in LA-4 cells via their specific receptors.

Figure 7

Murine BMDM were stimulated with apoptotic (ApoJ), or necrotic (NecJ) Jurkat T cells for 20 h in the absence (a,d,e) or presence of 10 μM NS-398, 30 μM PD-146176 (b), or 30 μM Y-27632 (c). Conditioned medium (CM) was added to LA-4 cells in the presence of TGF-β1 for 48 (c) or 72 h (a,b). CM was added to LA-4 cells in the presence of TGF-β1, with or without antagonists of EP2 (10 μM AH-6809), EP4 (10 μM AH-23848), DP1 (10 μM BW-A868C), DP2 (10 μM BAY-u3405), or c-Met (250 nM PHA-665752) for 48 (d) or 72 h (e). Immunoblots of total cell lysates were performed with anti-E-cadherin, N-cadherin, or α-SMA antibodies. Right: Densitometric analysis of the indicated EMT markers’ relative abundances. Values represent the mean ± s.e.m. of three independent experiments. *P < 0.05 compared with control; +P < 0.05 as indicated.