Figure 4. The mRNA expression level of Vtg, ERα, ARα, and ARβ˛mRNA in female and male mosquitofish from the Shima and Danshui Rivers as well as the reference site.

Error bars represent the standard deviations of the measured values. The mRNA expression of each target gene was compared to that in the reference site (S0). Significant differences between the sampling sites and reference site (S0) are indicated by an asterisk (p < 0.05).