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. 2016 Feb 15;6:21594. doi: 10.1038/srep21594

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Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Panel (A) illustrates ¹H-NMR spectra for the peptide containing L-α-Asp62 immediately after and 16, 20, 24, 28, 32, 35, and 38 h after heating at 70 °C (from top to bottom). Panel (B) shows ¹H-NMR spectra for 61–67 peptide containing D-β-Asp62 immediately after and 23, 26, 30, 32, 34, 36, and 38 h after heating at 70 °C. Each panel shows Asp62 H_α (5.2–4.8 ppm) and Thr63 methyl regions (1.8–1.4 ppm). In panel (C), the H_α region of L-α- and D-β-Asp62 immediately after (in black) and 38 h after heating (in red) is expanded in upper and lower traces, respectively.