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. 2016 Feb 15;6:21594. doi: 10.1038/srep21594

Figure 6. Real time NMR signal intensity change accompanied by the peptide bond cleavage at L-α- and D-β-Asp62 of αB-crystallin 61–67 analog.

Figure 6

The increase in the product N-terminal Thr63 (Δ) of 63–67 fragment from the reactant L-α-Asp 61–67 is plotted in (a), together with the increase in N-terminal Thr63 (Inline graphic) from the reactant D-β-Asp 61–67. Each symbol designates the integral intensity of the respective signal. In (b), the decrease of the reactant evaluated from the integral intensities of non-terminal L-α-Asp62 (Inline graphic) and Thr63 (Inline graphic) in L-α-Asp 61–67 is shown, as well as that of non-terminal D-β-Asp62 (Inline graphic) and Thr63 (Inline graphic) in D-β-Asp 61–67. All values are relative to the initial intensities. Black and red solid lines in (a) are the resulting curves obtained by fitting equation 2 to the respective experimental values, from which the rate constants k were estimated. Dashed lines in (b) are only guide for eyes.