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. 2016 Feb 15;6:20847. doi: 10.1038/srep20847

Figure 4. Representative examples of HPV16 E2 disruption in benign cervix samples.

Figure 4

HPV16 human foreskin keratinocytes (HFK) and SiHa represent amplification from HPV16 episomal and integrated controls; an intact E2 gene in primary HFK transfected with episomal forms of HPV16; a disrupted E2 gene in the HPV16 positive cervical cell line, SiHa. Results from each of the 7 overlapping primer sets and housekeeping gene (GAPDH) are shown for each of the 4 benign cervix samples (BC 55, BC 77, BC 79 and BC 83); with the E2 gene disrupted in all 4 patient samples. A template negative PCR reaction served as a negative control. * Multiple bands are sometimes observed if the concentration of DNA going into the second PCR reaction is high.