Table 2. Concordance of results from HPV testing using all three methods.
Ventana Positive N = 18 | Ventana Negative N = 17 | ||
---|---|---|---|
ISH+ Luminex+ Q-PCR+ | 5 | ISH− Luminex− Q-PCR− | 13 |
ISH+ Luminex+ Q-PCR− | 2 | ISH− Luminex− Q-PCR+ | 4 |
ISH+ Q-PCR+ Luminex− | 9 | ISH− Q-PCR− Luminex+ | 0 |
ISH+ Q-PCR− Luminex− | 2 | ISH− Q-PCR+ Luminex+ | 0 |
A total of 55 samples were tested for HPV using all three methods, with 35 samples providing an evaluable result. 18 cases tested HPV positive using Ventana ISH; 5 of these were positive using all three detection methods; 2 were positive using ISH and Luminex but negative using Q-PCR, 9 were positive using ISH and Q-PCR but negative using Luminex; 2 were positive using ISH but negative using Luminex and Q-PCR. 17 cases tested HPV negative using Ventana ISH; 13 of these were negative using all three detection methods; 4 were negative using ISH and Luminex but positive using Q-PCR.