Figure 5. Treatment of macrophages with anti-vimentin antibody increases ROS level and killing of M. tuberculosis.
Uninfected macrophage monolayers were treated with anti-vimentin antibody or normal IgG and ROS was measured as DCFDA fluorescence. (a) at 6 h; n = 8, p = 0.005 and (b) at 24 h; n = 7, p = 0.004. (c) Macrophage monolayers were infected with H37Rv and treated with anti-vimentin antibody or normal IgG after phagocytosis. After 24 h post infection, the cells were lysed, diluted to 10−2 and plated; n = 8, p = 0.034. Values are expressed as mean ± SE. CFU measurements show that anti-vimentin antibody treatment decreases the number of live H37Rv.