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. 2016 Feb 15;6:21076. doi: 10.1038/srep21076

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Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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XAS spectra across the Ca L_2,3-edge of Mytilus edulis shell grown under present day conditions for (a) the outer calcite, (b) the interface calcite, (c) the interface aragonite and (d) inner aragonite area. The colour of the present day (380 μatm pCO₂) solid line corresponds to the area with the same colour circle in Fig. 1. The spectra for each region of the shell grown under OA (1000 μatm pCO₂) conditions are shown as the solid black line in each panel. The spectra have been overlaid to highlight the increased ACC presence in shells grown under OA in the areas determined to be calcite and aragonite by XPEEM and EBSD. The six features in the XAS spectra are labelled 1–6. The solid vertical line in (a) indicates the position of the lower energy shoulder indicative of ACC. The dashed vertical line in (a) indicates the position of the peak that increases intensity from the outer calcite to the inner aragonite layer.