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. 2016 Feb 15;6:21664. doi: 10.1038/srep21664

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Copyright © 2016, Macmillan Publishers Limited

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(A) Representative immunoblot for CDK2 of LPS-treated podocytes with or without GIT27 treatment. Tubulin is included as a loading control. (B) Quantification of CDK2 shows that co-treatment of podocytes with LPS and GIT27 prevents downregulation of CDK2. The experiment was performed three times with three replicates in each experiment. Data are presented as mean ± SD. *p < 0.05 vs. LPS group. (C) Flow cytometry of podocytes stained for Annexin V confirms that co-treatment of podocytes with GIT27 and LPS prevents induction of apoptosis. The experiment was performed three times with three replicates in each experiment. Data are presented as mean ± SD. ***p < 0.001, ****p < 0.0001 vs. LPS group. (D) Representative immunoblot for CDK2 in cultured human podocytes treated with sera with low or high LPS activity, and with or without GIT27 co-treatment. Tubulin is included as a loading control. (E) Quantification of CDK2 from podocytes treated with sera with high or low LPS activity (n = 6 each), or treated with sera with high LPS activity in the presence of GIT27 (n = 6). The expression of CDK2 was lower after treatment with sera with high LPS activity than with low LPS activity. GIT27-treatment prevented downregulation of CDK2 induced by high LPS activity. The experiment was performed three times. Data are presented as mean ± SD. *p < 0.05, vs. high LPS group. (F) Quantification of In-Cell Western of cleaved caspase-3 in podocytes treated with sera with high or low LPS activity with or without GIT27 treatment shows that the expression of cleaved caspase-3 was higher after treatment with high-LPS sera compared to treatment with low-LPS sera. GIT27-treatment prevented the induction of apoptosis. DRAQ5^TM was used for normalization. Treatments were performed with sera from individual patients (n = 6 per group). The experiment was performed three times with 32 replicates in each group. Data are presented as mean ± SD, ****p < 0.0001 vs. high LPS group.