Figure 4. POU domain of Oct4 is the minimal requirement for generating iPS cells.

(a) The number of GFP+ iPSCs colonies formed by mOct4 WT, the indicated deletion mutants, or the YAP^TAD fusion proteins was calculated. Data are representative of three independent experiments. Statistics analysis was performed using a t-test (mean and s.d. of duplicate assays). (b) The relative transcriptional activities of mOct4 WT, the indicated deletion mutants, or the YAP^TAD fusion proteins were measured using 6xCR4-Luc and 5xW-Luc reporters, respectively. Data are representative of at least three independent experiments. Statistics analysis was performed using a t-test (mean and s.d. of duplicate assays). (c) The relative transcriptional activities of mOct4 WT, POU, POU-YAP^TAD and YAP^TAD were measured using 6xCR4-Luc and 5xW-Luc reporters, respectively. Data are representative of at least three independent experiments. Statistics analysis was performed using a t-test (mean and s.d. of duplicate assays). (d) The number of GFP+ iPSC colonies formed by increasing doses of mOct4 WT and POU-YAP^TAD was calculated. The corresponding protein levels of Oct4 WT and POU-YAP^TAD were shown in the lower panel. *:non-specific band (N.S.). (e) iPSC colony formed by POU-YAP^TAD under GFP fluorescence and phase contrast. Scale bars, 100 μm. (f) Establishment of iPS cell lines generated by POU-YAP^TAD. Scale bars, 250 μm.