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. 2016 Feb 15;6:21584. doi: 10.1038/srep21584

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Copyright © 2016, Macmillan Publishers Limited

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(A) P-selectin-Fc:ProG-TGTs were assembled and immobilized on the inner surface of a flow chamber where leukocytes were flowed through. A single leukocyte location was captured by consecutive dark field imaging with 3 sec time interval. Yellow arrow indicates flow direction. (B) Single leukocyte location with 0.3 sec time interval on control surface without P-selectin coating. Scale bar: 20 μm. (C) Velocity distribution. HL-60 cells attached onto P-selectin-Fc:ProG-TGTs and show rolling behavior. This resulted in a cell moving rate significantly lower than the rate on control surface. (D) DLL1-Fc:ProG-TGTs were assembled and immobilized on glass surface where Notch activation was tested. Notch receptors were activated on both T_tol = 12 pN surface and T_tol = 54 pN surface after incubation for two days. Notch activation was reported by H2B-YFP expression in CHO-K1 cell nucleus. Scale bar: 50 μm.