Figure 2. PISEMA spectra of [¹⁵N₅]-hΦ19W carrying five consecutive ¹⁵N labels in uniaxially oriented POPC bilayers.

(A) ambient temperature (295K), (B) 253K and (C) 223K. (D) nominal 100K (actual sample temperature due to microwave irradiation ~180K). (A–C) 10 mg of peptide were reconstituted at a peptide-to-lipid ratio (P/L) of 1/35 mole/mole supported by ultra-thin glass plates⁴⁷. (D) DNP/solid-state NMR spectrum of 8.5mg [¹⁵N₅]-hΦ19W in the presence of 0.3 mg AMUPol¹⁸ at P/L = 1/20 oriented onto films of high-density polyethylene³¹,³². The simulations show the intensity distributions at static helical tilt angles of 10^o (red circle) and 22^o (blue circle). Averaging around the helix long axis results in the collapse of the wheel -like pattern into its respective center of gravity (green dots).