Table 7. Physicochemical and in Vitro Safety Characterisation of Compounds 18 (RO5256390) and 48 (RO5263397).

parameter	RO5256390	RO5263397
basic pKa	8.98	8.07
Aq. solubilitya (μg/mL)	>4980	5830
logD at pH 7.4	1.29	1.12
PAMPA25 Peff (10–6 cm/s)	10.7	14.5
hERG IC20 (μM)	9.2	10.0
CYP 3A4/2D6/2C9 IC50 (μM)	>50/3.6/>50	>50/32/>50
AMES/MNT	NEG/NEG	NEG/NEG
in vitro phototoxicity26	NEG	NEG

^a0.05 M phophate buffer, pH = 7.2–7.5, thermodynamic sol.