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. 2016 Feb 15;14:12. doi: 10.1186/s12915-016-0234-9

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© Nakahara et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Olfr692 is expressed in a developmentally regulated manner, with maximum expression in adults older than 2 months. (a) Chromogenic in situ hybridization to investigate the temporal pattern of Olfr692 expression in the VNO during postnatal development (P0 to P60). At each age, images are representative from a set of 32 sections, from four individuals. (b, c) Temporal expression of V2R receptors in subclade A8 (b), investigated with probe for receptor gene Vmn2r107, and subclade A5 (c), investigated with probe for Vmn2r69 (images are representative from a set of 24 sections, from six individuals). lu, VNO lumen; spt, nasal septum. Scale bars represent 100 μm; panels without scale bars have the same magnification as the top rightmost panel. Blue labeling indicates DAPI nuclear staining