Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Feb 15;14:12. doi: 10.1186/s12915-016-0234-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Nakahara et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Fig. 6 — Olfr692 cells are activated by juvenile odors in a sexually dimorphic manner and depending on the individual’s social status. (a–j) Double fluorescent in situ hybridization for receptor gene Olfr692 (green) and immediate-early gene Egr1 (red), marker of vomeronasal neuron activation, in adult C57BL/6 virgin males (b), virgin females (d), parenting fathers (h) and mothers (j), exposed to same-strain pup. For h and j, exposure was to alien pups. Insets in b show higher magnification images of activity in Olfr692 cells in virgin males. Virgin males, virgin females, fathers and mothers were also exposed to control odors (a, c, g and i). Lactating female C57BL/6 mice (e) or milk extracted from lactating females (e') activate the VNO of adult male mice (Egr1 staining in red), as compared to control non-exposed animals (a). However, activated cells do not express Olfr692 (green fluorescence). (f) Egr1 expression (red) shows that recombinantly expressed ESP22 juvenile peptidic pheromone does not activate Olfr692 cells (green) in adult C57BL/6 males (right); exposure to MBP, the purification label for ESP22, does not activate the VNO (left). (k) Quantification of labeled cells in experiments shown in a–h. Left panel: percentage of Olfr692 labeled cells per section that are also Egr1-positive (n is the number of mice; * P = 0.0001, two-sample Welch’s t-test assuming unequal variances, t = 5.136; ** P = 0.005, Welch’s t-test, t = 4.407; see also Additional file 7: Table S1 for percentage of Egr1-positive cells among all counted Olfr692 labeled cells). Middle panel: number of Egr1/Olfr692 doubly labeled cells per VNO section (n is the number of mice; *** P = 0.02, two-sample Welch’s t-test, t = 2.474; **** P = 0.02, Welch’s t-test, t = 2.509). Right panel: total number of Olfr692-positive cells per VNO section (n is the number of sections; n.s. = statistically non-significantly different, two-sample t-test, P = 0.461, t = 0.099). Microscopy images are representative from the set of scored sections indicated in Additional file 7: Table S1. Quantification of activation in all experiments is detailed in Additional file 7: Table S1. lu, VNO lumen. Scale bar = 100 μm. In graphs, mean ± SEM