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. 2016 Feb 15;13:39. doi: 10.1186/s12974-016-0499-5

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© Hines-Beard et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Treatment with rAAV.EpoR76E altered microglial morphology. a–f Representative high-magnification confocal micrographs of IBA-1 immunolabeled (green) microglia in 3-month-old (a) and 8-month-old (b–f) retinas. These morphologies were present in retinas from both treatment groups. g, h Box and whisker plots of microglial soma area of cells from the central (g) or peripheral (h) retina, ****p < 0.0001. i, j Box and whisker plots of microglial ramification number for cells from the central (i) or peripheral (j) retina, *p < 0.05. k, l Scatter graphs of microglial soma area versus soma number in the central (k) or peripheral (l) retina. m, n Scatter graphs of microglial soma area versus ramification number in the central (m) or peripheral (n) retina