Figure 6.

Effect of hUCMSCs and hFFs on the invasion ability of HTR-8/SVneo cells. (a) HTR-8/SVneo cells (4 × 10⁴ cells/well) were cultured in medium containing 0, 25, 50, or 100% hUCMSC (a1–a4) or hFF (b1–b4) supernatant in Transwell inserts for 24 hours or were cocultured with hUCMSCs (c1–c4) or hFFs (d1–d4) at a concentration of 0, 2.5, 5, or 10 × 10⁴ cells/well in Transwell inserts for 24 hours. Cells that had invaded were stained with calcein-AM. (b) HTR-8/SVneo cells that had invaded were counted after staining. ∗ represents significant differences versus control (P < 0.05); ∗∗ represents very significant differences versus control (P < 0.01); # represents significant differences between hUCMSCs and hFFs at an individual dose (P < 0.05); ## represents very significant differences between hUCMSCs and hFFs at an individual dose (P < 0.01).