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. Author manuscript; available in PMC: 2016 Feb 15.
Published in final edited form as: Nat Protoc. 2013 Jan 3;8(1):213–222. doi: 10.1038/nprot.2012.152

Figure 3.

Figure 3

Real-time visualization of membrane fission. (a) SUPER templates are applied to a BSA-coated Lab-Tek chamber or coverslip for microscopy-based assays. Dynamin is added from a pipette to initiate the reaction. (b) SUPER templates are added to a well of a Lab-Tek chamber. (c) Spread SUPER templates evenly throughout the well by slowly moving the pipette according to the schematic while emptying the tip. (d) Once the templates are settled, transfer the well onto a microscope stage, begin to record a video and carefully add dynamin to the well.