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. Author manuscript; available in PMC: 2016 Feb 15.
Published in final edited form as: Nat Protoc. 2013 Jan 3;8(1):213–222. doi: 10.1038/nprot.2012.152

Figure 5.

Figure 5

Fission of membrane tethers. (a) SUPER templates are applied to a BSA-coated Lab-Tek chamber. After the templates settle, untreated silica beads of sizes (diameter = 20 μm) bigger than those used to produce the SUPER templates are added to the microscopy chamber in order to create tethers. Owing to their size, the 20-μm beads readily settle within the pipette tip. (b) Only the volume of the mixture that contains the settled beads is added into one corner of the well. (c) When they are first added to the wells and rolled over the templates, the larger beads clump. (d) Gently roll the clumped larger beads over the templates another three or four times by tilting the chamber in a W-shaped path. (Supplementary Video 4).